HPLC Method for Analysis of Omeprazole on Obelisc R Column

https://sielc.com/determination-omeprazole-on-obelisc-r-column
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Chromatogram

Method Parameters

Mobile PhaseGradient MeCN -20-50% in 15 min + 5 min hold
BufferAmmonium Acetate  pH 5.5 – 10 mM
Flow Rate1.0 ml/min
DetectionUV 300 nm
Class of CompoundsDrug
Analyzing CompoundsOmeprazole

HPLC Column Used

Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
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