Determination N-Acetyl-L-Cysteine on Primesep SB Column

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Chromatogram

Method Parameters

Mobile PhaseMeCN – 50%
BufferNa2HPO4 pH 4.0 – 50 mM
Flow Rate0.5 ml/min
DetectionUV, 200 nm
Class of CompoundsNutritional supplement, Hydrophilic, Ionizable
Analyzing CompoundsN-Acetyl-L-cysteine

HPLC Column Used

Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended