Determination Imatinib Mesylate on Obelisc R Column
https://sielc.com/determination-imatinib-mesylate-on-obelisc-r-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Imatinib mesylate .
Imatinib mesylate is a classified as a signal transduction inhibitor with the chemical formula C 29 H 31 N 7 O . It is an oral targeted therapy medication that is effective in treating chronic myeloid leukemia and metastatic gastrointestinal stromal tumors. Commercial versions of the drug were a topic of controversy when cancer specialists published a letter condemning the high costs of cancer treatments.
Imatinib mesylate can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Imatinib mesylate can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by UV at 270nm, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN -50% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Imatinib mesylate |