HPLC Method for Analysis of Atorvastatin
https://sielc.com/atorvastatinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Atorvastatin , Lipitor
Atorvastatin is a prescription medication that has C 33 H 35 FN 2 O 5 as its molecular formula. It is typically used to lower cholesterol, therefore reducing the risk of heart disease. Grapefruit juice can interfere with this medication.
Atorvastatin , Lipitor can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 – 0.1 % |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Statin, Hydrophobic, Ionizable |
| Analyzing Compounds | Atorvastatin,Lipitor |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended