Alltesta HPLC Method for Analysis of Linear PEI + (Cu) on PEI Column
https://sielc.com/alltesta-hplc-determination-of-peiChromatogram
Description
· Separation type: Liquid Chromatography Reversed-phase · HPLC Method for Analysis of Polyethylenimine PEI on PEI Column by SIELC Technologies
Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 275nm
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60%, |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Polymer, Basic, Hydrophilic, Ionizable |
| Analyzing Compounds | Polyethylenimine PEI |
HPLC Column Used
PEI, 4.6 x 250 mm, 5 µm, 100 A, dual ended