Zn and Na Quantitation in Drug Substances
https://sielc.com/Application-Zn-and-Na-Quantitation-in-Drug-SubstancesChromatogram
Description
Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Zinc |
HPLC Column Used
Primesep 100, 4.6×250 mm, 5 µm, 100A