USP Methods for the Analysis of Niacin with the Legacy L1 Column
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Description
Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.
Method Parameters
| Mobile Phase | Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2 |
|---|---|
| Buffer | Hexanesulfonate |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamin B3, Niacin |
HPLC Column Used
Legacy L1, 4.6×150 mm, 5 µm, 100A