USP Methods for the Analysis of Naproxen Using Legacy L1 Column
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Description
Application Notes: Naproxen is a common NSAID used for pain relief and fever reducer. According to the USP methods, naproxen contains no less than 98% and no more than 101.5 percent naproxen calculated on a dried basis. The USP HPLC method for the separation of naproxen was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column · Application compounds: naproxen and benzophenone · Mobile phase: water/MeCN/Glacial Acetic acid (49/50/1) · Detection technique: UV · Reference: USP35: NF30
Method Parameters
| Mobile Phase | H2O/MeCN/Glacial Acetic acid (49/50/1) |
|---|---|
| Buffer | Glacial Acetic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 254 nm |
| Class of Compounds | Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Naproxen, Benzophenone |