USP Methods for Chloramphenicol using a Legacy L1 Column
https://sielc.com/Application-USP-Methods-for-Chloramphenicol-using-a-Legacy-L1-ColumnChromatogram
Description
Application Notes: Chloramphenicol is a common broad spectrum antibiotic developed in the 1940’s. According to the USP methods, chloramphenicol should contain no less than 97.0% and no more than 103% of chloramphenicol calculated on a dried basis. The USP HPLC method for the analysis of chloramphenicol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A.
Application Columns: Legacy L1 C18 HPLC column · Application compounds: Chloramphenicol · Mobile phase: MeOH/H2O/AcOH 45/55/0.1 · Detection technique: UV · Reference: USP 30: NF35
Method Parameters
| Mobile Phase | MeOH/H2O/AcOH 45/55/0.1 |
|---|---|
| Buffer | AcOH |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Chloramphenicol |
HPLC Column Used
Legacy L1, 4.6×150 mm, 5 µm, 100A