USP Method for the Analysis of Amoxicillin using the Legacy L1 Column
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Description
Application Notes: Amoxicillin is one of the most commonly prescribed antibiotics. It is often used for treating strep throat. According to USP methods, amoxicillin should not contain less than 900ug and no more than 1050ug of amoxicillin per mg. The USP HPLC method for the separation of amitriptyline was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.
Application Columns: Legacy L1 C18 HPLC column · Application compounds: Amoxicillin · Mobile phase: MeCN/50 mM dibasic sodium phosphate · Detection technique: UV · Source: USP35: NF30
Method Parameters
| Mobile Phase | MeCN/50 mM NaH2PO4 pH 5.0 (4/96) |
|---|---|
| Buffer | NaH2PO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds | Drug, Antibiotics, Hydrophobic, Ionizable |
| Analyzing Compounds | Amoxicillin |