HPLC Separation of Phthalic Acids using Hydrogen Bonding
https://sielc.com/The_Separation_of_Phthalic_Acids_using_Hydrogen_BondingChromatogram
Description
Phthalic acid, isophthalic acid and terephthalic acid are all isomers of each other. Being structurally similar, they can present difficulties to reverse-phase HPLC separation. Methods that require high organic concentrations in the mobile phase can cause dewetting in many reverse-phase columns. SHARC 1 column can be operated in anhydrous conditions and uses hydrogen bonding as the mechanism of separation. Here, phthalic acids were separated in pure acetonitrile (ACN), with the ability to adjust retention times by adding methanol (MeOH) to the mobile phase with formic acid and ammonium formate as buffer, making the method MS-compatible. Can also be UV detected at 270nm.
Method Parameters
| Mobile Phase | MeCN/MeOH |
|---|---|
| Buffer | AmFm, Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Phthalic acid, Terephthalic acid, Isophthalic acid |
HPLC Column Used
Sharc 1, 3.2×100 mm, 5 µm, 100A