HPLC Separation of Pseudoephedrine, Norephedrine, Phenylephrine, and Norphenylephrine Using Hydrogen Bonding Mode
https://sielc.com/Separation_of_Pseudoephedrine_Norephedrine_Phenylephrine_and_NorphenylephrineChromatogram
Description
Application Notes : Neurotransmitters are polar basic compounds. Several HPLC techniques are used to analyze these hydrophilic molecules. The most common techniques are reversed-phase with ion-pairing reagents chromatography, ion chromatography, HILIC, and mixed-mode chromatography. SIELC developed a new approach of analysis, which is based on hydrogen bond interactions. Hydrogen-bonding offers unique and alternative selectivity to traditional approaches like reversed-phase, HILIC, ion-exchange, and mixed-mode chromatography. Our methods are fully compatible with ELSD, LC/MS and prep chromatography.
Application Columns : SHARC 1, 3.2×100 mm, 5 um, 100A. To learn more about SHARC 1 columns click here . To order this column click here . To see more chromatographic separations check our web site.
Application compounds : Pseudoephedrine, norephedrine, phenylephrine and norphenylephrine · Detection technique : UV, LC/MS
Method Parameters
| Mobile Phase | MeCN/MeOH |
|---|---|
| Buffer | AmFm, Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Pseudoephedrine, Norephedrine, Phenylephrine, Norphenylephrine |