Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode
https://sielc.com/Separation_of_Oxytetracycline_and_Chlortetracycline_in_Mixed-ModeChromatogram
Description
Oxytetracycline and chlortetracycline are polar compounds related to broad-spectrum of tetracycline group of antibiotics. Slight hydrophobicity and presence of the basic group in both compounds allow to employ mixed-mode reversed-phase cation-exchange mechanism for separation of these compounds. Method can be used for analysis of antibiotics and related impurities with LC/MS compatible conditions.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Oxytetracycline, Chlortetracycline |
HPLC Column Used
Primesep 100, 4.6×150 mm, 3 µm, 100A