Separation of Acetaminophen and 4-Aminophenol on Primesep C
https://sielc.com/Separation_of_Acetaminophen_and_4-Aminophenol_on_Primesep_CChromatogram
Description
Acetaminophen and 4-Aminophenol ar both aminophenols. Acetominophen or paracetamol is a widely used analgesic and antipyretic. 4-Aminophenol is an intermediate in the synthesis of paracetamol and used in photography as a developer. Both compounds were retained and resolved on Primesep C. Primesep C is a mixed-mode HPLC column which uses reversed-phase and unique complex-forming groups to resolve compounds that might otherwise coelute.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | TEAPh |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable |
| Analyzing Compounds | Acetaminophen, 4-Aminophenol |
HPLC Column Used
Primesep C, 4.6×150 mm, 5 µm, 100A