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Chromatogram

Description

Primesep 100 separates the divalent cations, zinc and manganese, from their counter ion, chloride. The cations are retained by cation exchange and the chloride anion is eluted by ion exclusion. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is evaporative light scattering detection (ELSD) compatible.

Method Parameters

Mobile PhaseMeCN/H2O
BufferAmAc pH 4.0
Flow Rate1.0 ml/min
DetectionELSD
Class of CompoundsIons,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsChloride, Magnesium, Zinc

HPLC Column Used

Primesep 100, 4.6×50 mm, 5 µm, 100A