Separation of Vitamins in Mixed-Mode HPLC
https://sielc.com/Application-Separation-of-Vitamins-in-Mixed-Mode-HPLCChromatogram
Description
Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
HPLC Column Used
Primesep D , 4.6×150 mm, 5 µm, 100A