Separation of Thiabendazole on Mixed-Mode HPLC Columns
https://sielc.com/Application-Separation-of-Thiabendazole-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · High Performance Liquid Chromatography (HPLC) Method for Analysis of Thiabendazole.
Thiabendazole (TBZ) or tiabendazole is both a fungicide and parasiticide which goes by the trade names Mintezol and Tresaderm. As a fungicide, it controls mold and blight and as a parasiticide it can control various types of worms. According to the EURL-SRM (European Union Reference Laboratory – Single Residue Methods) TBZ is considered difficult to analyze by traditional multiresidue methods. We separated and analyzed TBZ using two mixed-mode columns with different modes of separation. Obelisc R has a long hydrophobic chain and multiple ion-pairing groups, and Primesep 100 contains acidic ion-pairing groups. Method is LC/MS compatible and can be use on many different pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Thiabendazole |