Separation of Sulphonium Ions in Polar Organic Mode
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Description
The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O – 90/10% |
|---|---|
| Buffer | TFA – 0.1% |
| Flow Rate | 1.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A