Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column
https://sielc.com/Application-Separation-of-Steroids-in-Reverse-and-Normal-Mode-on-Primesep-100-ColumnChromatogram
Description
The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both reversed- and normal-phase modes. Switching modes by changing the mobile phase reverses peaks 2 and 3. The separation method uses a mobile phase mixture of water and acetonitrile (MeCN, ACN) for reversed phase and hexane and ethanol for normal phase with UV detection at 240 nm.