Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column
https://sielc.com/Application-Separation-of-Sodium-Tetramethylammonium-and-Tetrabutylammonium-on-Primesep-200-ColumnChromatogram
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Description
Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc pH4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Quaternary amines |
| Analyzing Compounds | Sodium, Chloride, Tetramethylammonium, Tetrabutylammonium |
HPLC Column Used
Primesep 200, 4.6×100 mm, 5 µm, 100A