Separation of NyQuil Active Ingredients on Primesep C
https://sielc.com/Application-Separation-of-NyQuil-Active-Ingredients-on-Primesep-CChromatogram
Description
Primesep C separates the active ingredients in the over-the-counter cold medicine, NyQuil. Succinate and bromate co-elute early, but acetaminophen, pseudoephedrine, doxylamine, and dextromethorphan are baseline resolved. A combination of cation exchange, complex formation, and hydrophobic interactions separate this mixture with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 205 nm.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | TEAPh |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan |
HPLC Column Used
Primesep C, 4.6×150 mm, 5 µm, 100A