HPLC Separation of Mepiquat on Obelisc R Column
https://sielc.com/Application-Separation-of-Mepiquat-on-Obelisc-R-ColumnChromatogram
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Description
Mepiquat is a plant growth regulator that is monitored by EPA in water. It is a quaternary amine that is very polar in nature. Mepiquat and other hydrophilic amines are not retained on reversed-phase columns, and produce poor peak shape due to residual silanol interactions. Ion-pairing reagent is required to retain mepiquat. Ion-pairing reagents are not compatible with LC/MS detection and most environmental application require high sensitivity methods to determine very low level of contaminants. Obelisc R mixed-mode column can be used for EPA methods for determination of common pesticides, herbicides and insecticides where analysis of very polar ionic compounds is required.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Grow regulator, Hydrophilic, Ionizable |
| Analyzing Compounds | Mepiquat, Chloride |
HPLC Column Used
Obelisc R, 4.6×50 mm, 5 µm, 100A