Separation of Maleic and Succinic Acid on Primesep B2 column
https://sielc.com/Application-Separation-of-Maleic-and-Succinic-Acid-on-Primesep-B2-columnChromatogram
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Description
Organic and inorganic acids can be retained and separated on mixed-mode columns based on weak reversed-phase and weak/medium anion-exchange mechanisms. Amount of ACN, buffer concentration and buffer pH will affect retention time of organic and inorganic acids. Acids can be monitored by low UV, ELSD or LC/MS. Presence of ions is required to facilitate ion-exchange mechanism. Method can be used as a general approach for analysis of acidic hydrophilic and acidic hydrophobic compounds. Carboxylic acids along with inorganic acid can be retained and separated without ion-pairing reagent.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, UV 230nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Succinic Acid,Maleic Acid |
HPLC Column Used
Primesep B2, 4.6×50 mm, 5 µm, 100A