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Chromatogram

Method Parameters

Mobile PhaseMeCN
BufferH3PO4, H2SO4
Flow Rate1.0 ml/min
DetectionUV, 230 nm
Class of CompoundsAcid, Hydrophobic, Ionizable
Analyzing Compounds4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid

HPLC Column Used

Primesep 100, 4.6×150 mm, 5 µm, 100A