Separation of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose in Mixed-Mode Chromatography
https://sielc.com/Application-Separation-of-Guanosine-Diphosphate-Mannose-and-Guanosine-Diphosphate-Fucose-in-Mixed-Mode-ChromatographyChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose. Two nucleotides containing different sugar fragments were separated on Primesep SB mixed-mode HPLC column using LC/MS compatible conditions. Method can be used for analysis and isolation of guanosine diphosphate mannose and guanosine diphosphate fucose. Mechanism of retention is weak reversed-phase and strong anion-exchange. retention is controlled by buffer concentration and buffer pH. Both compounds exhibit strong interaction providing good retention and separation.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 2.9- 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Hydrophilic, Ionizable, Nucleotide sugar |
| Analyzing Compounds | Diphosphate Mannose, Guanosine Diphosphate Fucose |
HPLC Column Used
Primesep SB, 4.6×50 mm, 5 µm, 100A