Separation of Alginic Acid and Related Products
https://sielc.com/Application-Separation-of-Alginic-Acid-and-Related-ProductsChromatogram
Description
Alginate is used in various pharmaceutical preparations. Chemically, it is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. Alginic acid can be separated from benzoate, citric acid and saccharin by mixed-mode chromatography on Primesep C HPLC column. This method can be used to quantitate alginic acid, citric acid or saccharin in complex mixtures. Various detection technique can be used (UV, ELSD, LC/MS), based on mobile phase selection.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Na2HPO4 |
| Flow Rate | 0.25 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Alginic acid, Citric acid, Benzoic acid, Saccharin |
HPLC Column Used
Primesep C, 4.6×150 mm, 5 µm, 100A