HPLC Separation of Albuterol and Amoxicillin
https://sielc.com/Application-Separation-of-Albuterol-and-AmoxicillinChromatogram
Description
Primesep 100 separates albuterol and amoxicillin with baseline resolution by a combination of cation-exchange, hydrophobic, and polar interactions. The mass spec (LC/MS) compatible separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | TFA – 0.15% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Antibiotics, Hydrophobic, Ionizable |
| Analyzing Compounds | Albuterol, Amoxicillin |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A