HPLC Separation and Retention of Vitamins in Mixed-Mode on Primesep B2
https://sielc.com/Application-Separation-and-Retention-of-Vitamins-in-Mixed-Mode-HPLC-Primesep-B2Chromatogram
Description
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
HPLC Column Used
Primesep B2, 3.2×50 mm, 5 µm, 100A