Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode
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Description
A general approach for analysis of various organic acids with MS detection in negative mode was developed using hydrogen-bonding stationary phase – SHARC 1. A highly sensitive method allows to analyze traces of organic acids in various matrices using ACN/MeOH/ammonia mobile phase. In hydrogen-bonding chromatography acetonitrile is a weaker solvent and alcohol is a stronger solvent. Various gradient and isocratic conditions can be used
Method Parameters
| Mobile Phase | MeCN/MeOH – 95/5% |
|---|---|
| Buffer | Ammonia 10mM |
| Flow Rate | 005 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Benzoic Acid, Acetylbenzoic acid |
HPLC Column Used
Sharc 1, 3.2×100 mm, 5 µm, 100A