Separation of Antibiotics in Mixed-mode chromatography
https://sielc.com/Separation%20of%20Antibiotics%20in%20Mixed-mode%20chromatographyChromatogram
Description
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
Method Parameters
| Mobile Phase | Gradient MeCN – 0-25%, 6 min, 25-70% 14 min |
|---|---|
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A