Separating Triadimefon from Impurities on Primesep 100 and Obelisc R HPLC Columns
https://sielc.com/Application-Separating-Triadimefon-from-Impurities-on-Primesep-and-Obelisc-HPLC-ColumnsChromatogram
Description
Triadimefon is known as Bayleton, usually used in a mixture of pesticides including captan, and folpet. A triazole family pesticide, triadimefon is a systemic fungicide that controls the mildew and fungal pests on trees, turf and fruits. It is considered moderately toxic and a method is useful for analyzing triadimefon. Primesep 100 and Obelisc R were the mixed-mode columns used. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R uses a long hydrophobic chain and multiple ion-pairing groups. Method is LC/MS compatible and can be use for dozens of pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Triadimefon |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A