Retention of Azide Ion on Obelisc N Column
https://sielc.com/Retention_of_Azide_Ion_on_Obelisc_N_ColumnChromatogram
Description
Sodium azide is a salt of weak hydrazoic acid with pKa 4.5-4.7. Sodium azide is very toxic. It is typically used as a stabilizer for aqueous solution to suppress bacteria grows. Chromatography separation is based on weak ionic interaction of the partially ionized hydrazoic acid with positively charged column surface. Additional polar interaction is a result of high acetonitrile concentration in the mobile phase.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Base, Hydrophobic, Ionizable |
| Analyzing Compounds | Azide |
HPLC Column Used
Obelisc N, 2.1×150 mm, 5 µm, 100A