Retention of the Herbicide Dicamba on Mixed-Mode HPLC Columns
https://sielc.com/Retention-of-the-Herbicide-Dicamba-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Dicamba is an organochloride derivative of benzoic acid. It is used as a non-specific herbicide that is usually used on non-crop plants. Dicamba is moderately toxic to humans, and both persistent and mobile in soils. The EURL (European Union Reference Laboratory) tested dicamba in an analysis of acidic pesticides. Primesep 100 and Obelisc R were used to separate dicamba with unique separation. Method is LC/MS compatible
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dicamba |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A