Retention Control of Bromacil on Primesep and Obelisc Mixed-Mode Columns
https://sielc.com/Application-Retention-Control-of-Bromacil-on-Primesep-and-Obelisc-Mixed-Mode-ColumnsChromatogram
Description
Bromacil is in a group of herbicides called substituted uracils. It non-selectively enters plant root systems and interferes with photosynthesis. Bromacil is used to control brush, but not used in cropland or residential areas because it is toxic. Primesep 100 and Obelisc R were used to separate bromacil with different selectivity. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep 100 contains embedded acidic ion-pairing groups. Method is LC/MS compatible and useful for separating many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Bromacil |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A