Phosphamidon Analysis Using Obelisc and Primesep Mixed-Mode HPLC Columns
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Description
Phosphamidon is an organophosphate insecticide commercially available as 70% Z -isomer and 30% E -isomer. It is highly toxic to mammals, and is classified by the World Health Organization to be extremely hazardous (Ia), with an oral lethal dose of less than 5mg/kg of bodyweight. The EURL included phosphamidon as a target pesticide for the EUPT-CF9. Primesep B2 and Obelisc R were used to retain phosphamidon and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing phosphamidon, other organophosphate insecticides, and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Phosphamidon |