Paraquat and Diquat Separation in Non-aqueous Mobile Phase
https://sielc.com/Paraquat_Diquat_Separation_in_Non-aqueous_Mobile_PhaseChromatogram
Description
Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)
Method Parameters
| Mobile Phase | MeCN/MeOH – 90/10% |
|---|---|
| Buffer | H2SO4 – 0.2 |
| Flow Rate | 3 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Paraquat, Diquat |
HPLC Column Used
Sharc 1, 4.6×100 mm, 5 µm, 100A