Nabam Analysis on Mixed-Mode HPLC Columns
https://sielc.com/Application-Nabam-Analysis-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Nabam is a di-thiocarbamate fungicide which is used on soils and leaves to protect from a variety of diseases on fruits, vegitables, field crops, and turf. Obelisc R and Primesep B2 were used to retain nabam and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing nabam and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Nabam |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A