Monocrotophos Separation on Obelisc and Primesep Mixed-Mode HPLC Columns
https://sielc.com/Application-Monocrotophos-Separation-on-Obelisc-and-Primesep-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Banned in the U.S. and many other countries, Monocrotophos is a cheap and toxic organophosphate insecticide. It is especially toxic to birds, being blamed for widespread bird kills. The EURL (European Reference Laboratory) included monocrotophos in a the EURL-FV a proficency test for multi-residue methods using QuEChERS (Quick, East, Cheap, Effective, Rugged, Safe) followed bby LC/MS/MS for fruits and vegitables. Primesep B2 and Obelisc R were used to retain monocrotophos and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing monocrotophos, other organophosphate insecticides, and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Monocrotophos |