Improved Peak Shape of a Basic Compound on Primesep B2 Column
https://sielc.com/Application-Improved-Peak-Shape-of-A-Basic-Compound-on-Primesep-B2-ColumnChromatogram
Description
Primesep B2 shows superior efficiency, symmetry, and peak shape for a hydrophilic basic compound compared to two leading reversed-phase C18 columns. Primesep B2 contains an embedded basic functional group that shields the underlying silica by ionic repulsion with the analyte. On reversed-phase columns exposed active silanols provide unintended polar interactions with basic analytes that lead to tailing. The HPLC separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Oxymetazoline |
HPLC Column Used
Primesep B2, 4.6×150 mm, 5 µm, 100A