Hydrophobic and Hydrophilic Compound Separation
https://sielc.com/Application-Hydrophobic-and-Hydrophilic-Compound-SeparationChromatogram
Description
Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Nonpolar, Polar, Supplements |
| Analyzing Compounds | Cysteine, L-cystine, 2,2-dimethylcystine, 2-methylcysteine, Benzoic Acid, Toluene |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A