HPLC Separation of Octopamine and Synephrine on Primesep 200 Column
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HPLC Separation of Octopamine and Synephrine on Primesep 200 Column
Description
Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable,Neurotransmitter |
| Analyzing Compounds | Octopamine, Synephrine |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A