HPLC Separation of Fungicides in a Green Tea Matrix
https://sielc.com/HPLC_Separation_of_Fungicides_in_Green_Tea_MatrixChromatogram
Description
Even though teas are known for containing polyphenols such as catechins, flavonoids and theaflavins, SIELC mixed-mode columns can be used to analyze a matrix from a commercial tea and retain target compounds. In this method, green tea was spiked with 0.005mg/ml of pesticides previously found in teas. Primesep 200 was used for it’s reverse-phase and acidic ion-pairing groups. Target compounds were retained and resolved from the majority of the tea matrix peak.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 – 0.05 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Statin, Hydrophobic, Ionizable |
| Analyzing Compounds | Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A