HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography
https://sielc.com/HPLC_Separation_of_Antibiotics_of_Group_in_Mixed-Mode_ChromatographyChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This m ethod is compatible with LC/MS and prep chromatography.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Josamycin, Norfloxacin, Marbofloxacin, Flumequine |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A