HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

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Chromatogram

Method Parameters

Mobile PhaseMeCN – 97.5%
BufferH3PO4 – 2.5%
Flow Rate3.0 ml/min
DetectionUV, 210 nm
Class of CompoundsDrug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine

HPLC Column Used

Sharc 1, 4.6×100 mm, 5 µm, 100A