HPLC Separation of Agricultural Compounds Found in Tea
https://sielc.com/HPLC_Separation_of_Agricultural_Compounds_Found_in_TeaChromatogram
Description
Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.
Method Parameters
| Mobile Phase | MeCN/H2O – 45/55%, MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Statin, Hydrophobic, Ionizable |
| Analyzing Compounds | Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A