HPLC Separation of a Complex of Quinolone Antibiotics
https://sielc.com/HPLC_Separation_of_A_Complex_of_Quinolone_AntibioticsChromatogram
Description
Quinolones are a group of synthetic broad-spectrum antibiotics with a wide variety of applications in medicine and veterinary sciences. Some quinolones have been found in food products including fish and shrimp. Primesep 100 was used to separate a mixture of quinolones and achieve baseline separation. Retention was controlled by adjusting acetonitrile in the mobile phase.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Quinolone Antibiotics, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Oxolinic Acid, Flumequine, Ciprofloxacin, Enrofloxacin, Pefloxacin |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A