HPLC Analysis of Ethephon in Hydrogen-Bonding Mode
https://sielc.com/HPLC_Analysis_of_Ethephon_in_Hydrogen-Bonding_ModeChromatogram
Description
Ethephon is an important compound for the agricultural industry. It can be corrosive, but metabolizes to ethylene quickly in plants. Ethephon was retained on SHARC 1 HILIC/anion-exclusion column. Retention was controlled by adjusting methanol in mobile phase to suppress hydrogen bonding.
Method Parameters
| Mobile Phase | Gradient MeCN/MeOH – 90/10% to 50/50, 6 min |
|---|---|
| Buffer | Ammonia – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Hydrophilic, Ionizable |
| Analyzing Compounds | Ethephon |
HPLC Column Used
Sharc 1, 3.2×100 mm, 5 µm, 100A