HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column
https://sielc.com/Application-HPLC-Separation-of-Vitamin-E-and-Vitamin-E-AcetateChromatogram
Description
Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 90/10% |
|---|---|
| Flow Rate | 0.5 ml/min |
| Detection | UV 205 |
| Class of Compounds | Vitamins, Hydrophilic, Ionizable |
| Analyzing Compounds | Vitamin E, Vitamin E Acetate |
HPLC Column Used
Primesep B, 3.2×150 mm, 5 µm, 100A