HPLC Separation of Vitamin B
https://sielc.com/Application-HPLC-Separation-of-Vitamin-BChromatogram
Description
Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.
Method Parameters
| Mobile Phase | MeCN/H2O -10/90% |
|---|---|
| Buffer | NEt3H3PO4 Ph 3.0 – 20 Mm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Pyridoxine, Thiamine, Riboflavin |
HPLC Column Used
Primesep C , 4.6×150 mm, 5 µm, 100A