HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids

https://sielc.com/Application-HPLC-Separation-of-Vanilmandelic-Hydroxyindoleacetic-and-Homovanillic-Acids
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Chromatogram

Method Parameters

Mobile PhaseMeCN
BufferTFA,  AmFm
Flow Rate1.0 ml/min
DetectionUV, 230 nm
Class of CompoundsAcid, Monocarboxylic acid,  Hydrophilic, Ionizable
Analyzing CompoundsVanilmandelic, Hydroxyindoleacetic, Homovanillic Acid

HPLC Column Used

Primesep D, 4.6×150 mm, 5 µm, 100A
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